The purpose of the BRAG program, Assistance Listing 10.219, is to support the generation of new information that will assist Federal regulatory agencies in making science-based decisions about the ecological effects of introducing genetically engineered (GE) organisms by techniques that use recombinant, synthesized, or amplified nucleic acids to modify or create a genome. The organisms include plants, microorganisms (including fungi, bacteria, and viruses), arthropods, fish, birds, livestock, and other animals. These include related wild and agricultural organisms.