This Notice of Funding Opportunity (NOFO) invites applications to usethe NIH INCLUDE Project (INvestigation of Co-occurring conditions across the Lifespan to Understand Down syndromE) to access resources to generate a large volume of integrated genomic and multi-omics data to facilitate discovery of the molecular mechanisms of health conditions related to Down syndrome. These data, and associated clinical and phenotypic data, will become part of the INCLUDE Project data resource for sharing with the research community. No funding will be provided directly to applicants under this NOFO. Applicants are encouraged to propose omics essays of existing biospecimens collected from individuals with Down syndrome and controls. The program will accept applications that propose whole genome sequencing, including both short-read and long-read, exome, epigenome, and transcriptome sequencing, as well as proteomic, metabolomic, and single-cell RNA sequencing and ATAC sequencing, when justified. The omics data and related phenotypic data will be deposited in a public INCLUDE Project controlled-access database.